El-Abd E2
faculty
Title: Pre-irradiation effects of ectoine on radiation-induced cardiotoxicity in female Swiss albino mice model
Biography
Biography: El-Abd E2
Abstract
Background and purpose: In vivo, ectoine single dose showed post-irradiation protective effects via modulation of inflammatory and oxidative stress pathways. The current study aims to explore the possible accumulative pre-irradiation protective effect(s) of ectoine.
Experimental approach: Forty female Swiss albino mice (20–22 gm) divided into four groups; controls (injected intraperitoneally for ten days with 0.2 ml saline), ectoine groups injected with 20 mg/kg of ectoine for ten days), irradiated groups (received six Gy whole body x-irradiation single dose then injected with saline for ten days), ectoine irradiated groups (injected with ectoine for ten days then irradiated). Animals sacrificed on day seven, and 14 (five animals each). Hearts examined for histological changes and immunostained for Bax. Ectoine concentration in hearts measured by HPLC. Serum cardiac troponin T (cTnT), total antioxidant capacity (T-AOC), and apoptosis inducing factor (AIF) evaluated by ELISA. Key results: Heart histological changes documented in 40% of the 7- & 14-days post irradiation. Ectoine concentrations ( mg/mg of heart weight) was higher in ectoine groups than ectoine irradiated groups ( mg/mg of heart weight) 14-days post treatment. Serum cTnT significantly differed between the 14 days groups (p = 0.032). Apoptosis inducible factor (AIF) significantly increased in ectoine irradiated group (at 14 days) than those of control (p = 0.014), irradiated (p = 0.020), and ectoine (p = 0.033) groups. Bax showed strong to moderate immunostaining in ectoine and irradiated groups.
Conclusion and implications: Ectoine have pre-irradiation accumulative effects on heart via modulation of apoptosis.